Hotspot

psbE-petL

LSC · 64600-65901 · 1302 bp

Back to Alternanthera

Pi 0.0301

MarkerSeek score 66.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1302
variable_sites: 39
pi: 0.0301
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCCGAGGTTATAGTTAAAGCC	50.0	45.5	57.9	57.9	1466-1487 bp	1.0	65.6
psbE-petL_p2	CAACCCGCAATGAATAGGGA	CGAGGTTATAGTTAAAGCCGC	50.0	47.6	57.9	56.9	1463-1484 bp	1.0	65.2
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGCCGAGGTTATAGTTAAAGCC	50.0	45.5	57.8	57.9	1389-1410 bp	1.0	64.9
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AGCCGAGGTTATAGTTAAAGCC	55.0	45.5	58.2	57.9	1384-1405 bp	1.0	64.6
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	CGAGGTTATAGTTAAAGCCGC	50.0	47.6	57.8	56.9	1386-1407 bp	1.0	64.5