Hotspot

ndhC-trnV

LSC · 51211-51688 · 478 bp

Back to Alternanthera

Pi 0.0318

MarkerSeek score 71.80

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 15
pi: 0.0318
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	TCCTTTGCTACTCGGAGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	616-622 bp	1.0	80.0
ndhC-trnV_p2	TTCTCGGGTCCTTTGCTACT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	624-630 bp	1.0	78.2
ndhC-trnV_p3	TTTCTCGGGTCCTTTGCTAC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	625-631 bp	1.0	77.4
ndhC-trnV_p4	TCCTTTGCTACTCGGAGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	615-621 bp	1.0	77.3
ndhC-trnV_p5	TCCTTTGCTACTCGGAGCTA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	604-610 bp	1.0	76.2