Hotspot

psbC-trnS

LSC · 35320-35590 · 271 bp

Back to Alternanthera

Pi 0.0406

MarkerSeek score 68.30

Primer available yes

Label psbC-trnS

Feature Metadata

label_name: psbC-trnS
feature_type: igs
region: LSC
length_bp: 271
variable_sites: 11
pi: 0.0406
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS_p1	CCTGTTCTTTCGATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.3	58.0	393-401 bp	1.0	80.0
psbC-trnS_p2	CCTGTTCTTTCGATGACCCC	GTTGGAGAGATGGCTGAGTG	55.0	55.0	58.3	58.0	397-405 bp	1.0	79.9
psbC-trnS_p3	CCTGTTCTTTCGATGACCCC	GCTGAGTGGTTGATAGCTCC	55.0	55.0	58.3	58.1	385-393 bp	1.0	79.7
psbC-trnS_p4	CCTGTTCTTTCGATGACCCC	GGCTGAGTGGTTGATAGCTC	55.0	55.0	58.3	58.1	386-394 bp	1.0	79.7
psbC-trnS_p5	CCTGTTCTTTCGATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.3	58.1	378-386 bp	1.0	79.7