Hotspot

petN-psbM

LSC · 28606-29582 · 977 bp

Back to Alternanthera

Pi 0.0351

MarkerSeek score 74.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 977
variable_sites: 34
pi: 0.0351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	AAAAACGGTCAGCCAAAACG	50.0	45.0	57.6	58.1	1022-1024 bp	1.0	79.8
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	ACGGTCAGCCAAAACGATTA	50.0	45.0	57.6	57.8	1018-1020 bp	1.0	79.1
petN-psbM_p3	CCTGGGCTGCTTTAATGGTA	AAAAACGGTCAGCCAAAACG	50.0	45.0	57.6	58.1	1071-1073 bp	1.0	79.0
petN-psbM_p4	CCTGGGCTGCTTTAATGGTA	ACGGTCAGCCAAAACGATTA	50.0	45.0	57.6	57.8	1067-1069 bp	1.0	78.3
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	AACGGTCAGCCAAAACGATT	50.0	45.0	57.6	58.7	1019-1021 bp	1.0	75.0