Hotspot

trnC-petN

LSC · 28011-28515 · 505 bp

Back to Alternanthera

Pi 0.0376

MarkerSeek score 71.70

Primer available yes

Label trnC-petN

Feature Metadata

label_name: trnC-petN
feature_type: igs
region: LSC
length_bp: 505
variable_sites: 19
pi: 0.0376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-petN_p1	TGTTCCCCAGTTCAAATCCG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	623-638 bp	1.0	78.8
trnC-petN_p2	TGTTCCCCAGTTCAAATCCG	TACCATTAAAGCAGCCCAGG	50.0	50.0	58.1	57.6	574-589 bp	1.0	78.4
trnC-petN_p3	ACTGCAAATCCTTGTTCCCC	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	635-650 bp	1.0	76.6
trnC-petN_p4	GCAAATCCTTGTTCCCCAGT	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	632-647 bp	1.0	76.6
trnC-petN_p5	TGCAAATCCTTGTTCCCCAG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	633-648 bp	1.0	76.6