Hotspot

atpH-atpI

LSC · 13934-14621 · 688 bp

Back to Alternanthera

Pi 0.0336

MarkerSeek score 71.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 23
pi: 0.0336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TACCTTGACCAACTCCAGGT	GCGAATCCATGGAAGGTCAT	50.0	50.0	57.9	58.0	802-811 bp	1.0	81.5
atpH-atpI_p2	TACCTTCTACAGCCTGTCCC	GCGAATCCATGGAAGGTCAT	55.0	50.0	58.1	58.0	829-838 bp	1.0	81.2
atpH-atpI_p3	GCAGTACCTTGACCAACTCC	GCGAATCCATGGAAGGTCAT	55.0	50.0	58.2	58.0	806-815 bp	1.0	80.8
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.9	57.9	831-840 bp	1.0	80.5
atpH-atpI_p5	TACCTTCTACAGCCTGTCCC	TTTTGCAACTTTAGCCGCAG	55.0	45.0	58.1	57.9	858-867 bp	1.0	80.2