Hotspot

atpF-atpH

LSC · 13239-13687 · 449 bp

Back to Alternanthera

Pi 0.0227

MarkerSeek score 70.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 10
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGACCCAAGAAAACGA	GCAGAGGGAAAAATACGAGGT	50.0	47.6	58.0	58.0	605-613 bp	1.0	74.1
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGAGGT	50.0	47.6	57.9	58.0	632-640 bp	1.0	73.3
atpF-atpH_p3	CCAGTGACCCAAGAAAACGA	AGCAGAGGGAAAAATACGAGG	50.0	47.6	58.0	57.7	606-614 bp	1.0	72.0
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	AGCAGAGGGAAAAATACGAGG	50.0	47.6	57.9	57.7	633-641 bp	1.0	71.3
atpF-atpH_p5	ACACCAAGCACTACACTGAG	GCAGAGGGAAAAATACGAGGT	50.0	47.6	57.5	58.0	682-690 bp	1.0	69.8