Hotspot

rps16-trnQ

LSC · 6367-6981 · 615 bp

Back to Alternanthera

Pi 0.0309

MarkerSeek score 68.00

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 615
variable_sites: 19
pi: 0.0309
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	688-701 bp	1.0	80.5
rps16-trnQ_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	699-712 bp	1.0	79.9
rps16-trnQ_p3	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	718-731 bp	1.0	79.6
rps16-trnQ_p4	TCATGTCCTTCAAGTCGCAC	TAAGGCATCGGGTTTTGGTC	50.0	50.0	58.2	58.2	729-742 bp	1.0	79.1
rps16-trnQ_p5	AAGTCGCACGTTGCTTTCTA	AGAAATCTAAGTGGGGCGTG	45.0	50.0	58.1	57.6	747-760 bp	1.0	77.7