Hotspot

trnL-CAA-ycf15

IRa · 160911-168716 · 7806 bp

Back to Alstonia

Pi 0.0628

MarkerSeek score 53.80

Primer available yes

Label trnL-CAA-ycf15

Feature Metadata

label_name: trnL-CAA-ycf15
feature_type: igs
region: IRa
length_bp: 7806
variable_sites: 37
pi: 0.0628
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.05
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf15_p1	TGAGGAGAGCTTTGTGGAGA	ATACCCGTCACATGCTTTCC	50.0	50.0	58.0	58.0	1309 bp	0.333333	52.9
trnL-CAA-ycf15_p2	AGAAGGGGTTGGAGTCGTTA	ATACCCGTCACATGCTTTCC	50.0	50.0	58.0	58.0	1191 bp	0.333333	52.9
trnL-CAA-ycf15_p3	TGAGGAGAGCTTTGTGGAGA	TCGTTCCAGTGAAAAGGCAA	50.0	45.0	58.0	58.0	1917 bp	0.333333	52.8
trnL-CAA-ycf15_p4	TGAGGAGAGCTTTGTGGAGA	TTCGTTCCAGTGAAAAGGCA	50.0	45.0	58.0	58.0	1918 bp	0.333333	52.8
trnL-CAA-ycf15_p5	CATTCCGATGTAGTGGTGCA	ATACCCGTCACATGCTTTCC	50.0	50.0	58.0	58.0	1210 bp	0.333333	52.8