Hotspot

rps12-psbB

LSC · 70326-72775 · 2450 bp

Back to Alstonia

Pi 0.237

MarkerSeek score 81.50

Primer available yes

Label rps12-psbB

Feature Metadata

label_name: rps12-psbB
feature_type: igs
region: LSC
length_bp: 2450
variable_sites: 742
pi: 0.237
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-psbB_p1	GGCTGTCTTGTATTTCTAATAAGTTGT	GGCCGGGATCATTCAATACA	33.3	50.0	58.1	57.7	2543-2707 bp	1.0	52.0
rps12-psbB_p2	GGCTGTCTTGTATTTCTAATAAGT	GGCCGGGATCATTCAATACA	33.3	50.0	54.7	57.7	2543-2707 bp	1.0	52.0
rps12-psbB_p3	GGCTGTCTTGTATTTCTAATAAGTTG	GGCCGGGATCATTCAATACA	34.6	50.0	56.7	57.7	2543-2707 bp	1.0	52.0
rps12-psbB_p4	GGCTGTCTTGTATTTCTAATAAGTT	GGCCGGGATCATTCAATACA	32.0	50.0	55.4	57.7	2543-2707 bp	1.0	52.0
rps12-psbB_p5	GGCTGTCTTGTATTTCTAATAAGTTGT	CGGCCGGGATCATTCAATAC	33.3	55.0	58.1	58.8	2544-2708 bp	1.0	52.0