Hotspot

ndhC-trnV-UAC

LSC · 51303-51977 · 675 bp

Back to Alstonia

Pi 0.11

MarkerSeek score 84.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 100
pi: 0.11
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTACCATAAAGAAGCGGCCA	GAGGTGCCTATTTAGGTGCC	50.0	55.0	58.0	58.0	1702-2434 bp	1.0	88.0
ndhC-trnV-UAC_p2	TGGTACCATAAAGAAGCGGC	GAGGTGCCTATTTAGGTGCC	50.0	55.0	58.0	58.0	1704-2436 bp	1.0	88.0
ndhC-trnV-UAC_p3	TCAAAGTCGAATCGTGACCC	GAGGTGCCTATTTAGGTGCC	50.0	55.0	57.9	58.0	1756-2488 bp	1.0	87.5
ndhC-trnV-UAC_p4	GTACCATAAAGAAGCGGCCA	GGTGCCAAATAGACCCTACG	50.0	55.0	58.0	58.1	1688 bp	0.333333	52.7
ndhC-trnV-UAC_p5	TGGTACCATAAAGAAGCGGC	GGTGCCAAATAGACCCTACG	50.0	55.0	58.0	58.1	1690 bp	0.333333	52.7