Hotspot

trnT-UGU-trnL-UAA

LSC · 47184-47950 · 767 bp

Back to Alstonia

Pi 0.0945

MarkerSeek score 89.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 97
pi: 0.0945
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 89.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	828-884 bp	1.0	89.0
trnT-UGU-trnL-UAA_p2	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	879-935 bp	1.0	88.1
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	860-916 bp	1.0	88.1
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	822-878 bp	1.0	87.7
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	860-916 bp	1.0	86.9