Hotspot

ycf3-trnS-GGA

LSC · 45258-45755 · 498 bp

Back to Alstonia

Pi 0.065

MarkerSeek score 87.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 498
variable_sites: 45
pi: 0.065
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 87.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTTATTCGCGATCTAGGC	CCCTCGGTAAACAAAAGCCT	47.6	50.0	57.0	58.1	560-604 bp	1.0	73.4
ycf3-trnS-GGA_p2	CCATTTATTCGCGATCTAGGC	TTCCAATGCTACGCCTTGAA	47.6	45.0	57.0	57.8	531-575 bp	1.0	73.0
ycf3-trnS-GGA_p3	CCATTTATTCGCGATCTAGGC	ACCCTCGGTAAACAAAAGCC	47.6	50.0	57.0	58.4	561-605 bp	1.0	71.1
ycf3-trnS-GGA_p4	TCCATTTATTCGCGATCTAGGC	CCCTCGGTAAACAAAAGCCT	45.5	50.0	58.4	58.1	561-605 bp	1.0	70.1
ycf3-trnS-GGA_p5	CCATTTATTCGCGATCTAGGC	AGTTCCAATGCTACGCCTTG	47.6	50.0	57.0	58.5	533-577 bp	1.0	70.1