Hotspot

trnC-GCA-petN

LSC · 28074-28948 · 875 bp

Back to Alstonia

Pi 0.0865

MarkerSeek score 84.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 106
pi: 0.0865
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CGCAGTCAGCAGTCAATAGT	ACTTCTTCCCCACACTACGA	50.0	50.0	58.0	58.0	1295-1317 bp	1.0	91.9
trnC-GCA-petN_p2	CGCAGTCAGCAGTCAATAGT	TTCTTCCCCACACTACGAGT	50.0	50.0	58.0	58.0	1302-1315 bp	0.666667	76.5
trnC-GCA-petN_p3	ATACTAGACGGGGATGGGTG	ACTTCTTCCCCACACTACGA	55.0	50.0	58.0	58.0	1203 bp	0.333333	53.2
trnC-GCA-petN_p4	ATACTAGACGGGGATGGGTG	TTCTTCCCCACACTACGAGT	55.0	50.0	58.0	58.0	1201 bp	0.333333	53.2
trnC-GCA-petN_p5	GGATGGGTGTGGATCCAATC	TTCTTCCCCACACTACGAGT	55.0	50.0	58.0	58.0	1190 bp	0.333333	53.2