Hotspot

rpoB-trnC-GCA

LSC · 26722-28002 · 1281 bp

Back to Alstonia

Pi 0.0781

MarkerSeek score 86.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1281
variable_sites: 140
pi: 0.0781
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 86.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCATTTCCATCCCCGAGC	CTTACCACTCGGCCATGC	57.9	61.1	58.0	58.2	1277-1324 bp	1.0	66.4
rpoB-trnC-GCA_p2	ATTCCCTCATTTCCATCCCC	CTTACCACTCGGCCATGC	50.0	61.1	56.9	58.2	1282-1329 bp	1.0	65.9
rpoB-trnC-GCA_p3	TCATTTCCATCCCCGAGCAT	CTTACCACTCGGCCATGC	50.0	61.1	59.2	58.2	1276-1323 bp	1.0	65.3
rpoB-trnC-GCA_p4	ACATTCCCTCATTTCCATCCC	CTTACCACTCGGCCATGC	47.6	61.1	57.7	58.2	1284-1331 bp	1.0	64.0
rpoB-trnC-GCA_p5	CTCATTTCCATCCCCGAGC	CCTTACCACTCGGCCATG	57.9	61.1	58.0	57.5	1278-1325 bp	1.0	63.6