Hotspot

ndhB

Genome · 1-1299 · 1299 bp

Back to Alsophila

Pi 0.0326

MarkerSeek score 59.30

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: Genome
length_bp: 1299
variable_sites: 72
pi: 0.0326
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	ATACATTAGGCGGGAGGGTT	CGTGGGAATCGCCTGAATAA	50.0	50.0	58.2	58.1	358-362 bp	0.6	62.6
ndhB_p2	ATACATTAGGCGGGAGGGTT	GGATTTACCTCCCCGCAAAT	50.0	50.0	58.2	57.9	273 bp	0.6	62.6
ndhB_p3	ATACATTAGGCGGGAGGGTT	TATGGCCTTTCTGGTGGAGA	50.0	50.0	58.2	58.0	684-688 bp	0.6	62.5
ndhB_p4	ATACATTAGGCGGGAGGGTT	GTATGGCCTTTCTGGTGGAG	50.0	55.0	58.2	58.0	685-689 bp	0.6	62.4
ndhB_p5	ATACATTAGGCGGGAGGGTT	GGCAGGAATGGCTTTCAAAC	50.0	50.0	58.2	57.9	577-581 bp	0.6	62.1