Hotspot

ycf1-chlN

Genome · 129613-129980 · 368 bp

Back to Alsophila

Pi 0.0518
MarkerSeek score 68.00
Primer available yes
Label ycf1-chlN

Feature Metadata

label_name
ycf1-chlN
feature_type
igs
region
Genome
length_bp
368
variable_sites
33
pi
0.0518
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-chlN_p1 CATCGATCCACTCAAGGCAA AAGGGGTTTATGTTTGCGGT 50.0 45.0 58.0 58.0 1222-1246 bp 1.0 84.4
ycf1-chlN_p2 ATCGATCCACTCAAGGCAAC AAGGGGTTTATGTTTGCGGT 50.0 45.0 58.0 58.0 1221-1245 bp 1.0 84.4
ycf1-chlN_p3 GATCCACTCAAGGCAACGAT AAGGGGTTTATGTTTGCGGT 50.0 45.0 58.0 58.0 1218-1242 bp 1.0 84.4
ycf1-chlN_p4 CATCGATCCACTCAAGGCAA GCGAAGGGGTTTATGTTTGC 50.0 50.0 58.0 58.0 1225-1249 bp 1.0 84.3
ycf1-chlN_p5 CATCGATCCACTCAAGGCAA ATGTTTGCGGTGTAAACCCT 50.0 45.0 58.0 58.0 1213-1237 bp 1.0 84.3