Hotspot

ndhI-ndhA

Genome · 120459-120618 · 160 bp

Back to Alsophila

Pi 0.086

MarkerSeek score 70.90

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: Genome
length_bp: 160
variable_sites: 24
pi: 0.086
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	GCTTGTTGCCCATAATCTCG	CATTTCAACTTCTGTTACTCAA	50.0	31.8	57.2	52.7	214-233 bp	1.0	52.0
ndhI-ndhA_p2	GCTTGTTGCCCATAATCTCG	CATTTCAACTTCTGTTACTCAAA	50.0	30.4	57.2	53.5	214-233 bp	1.0	52.0
ndhI-ndhA_p3	GCTTGTTGCCCATAATCTCG	CATTTCAACTTCTGTTACTCAAAT	50.0	29.2	57.2	54.1	214-233 bp	1.0	52.0
ndhI-ndhA_p4	GCTTGTTGCCCATAATCTCGA	CATTTCAACTTCTGTTACTCAA	47.6	31.8	58.7	52.7	214-233 bp	1.0	52.0
ndhI-ndhA_p5	GCTTGTTGCCCATAATCTCG	ATTTCAACTTCTGTTACTCAAAT	50.0	26.1	57.2	52.6	213-232 bp	1.0	52.0