Hotspot

ndhD-psaC

Genome · 118023-118175 · 153 bp

Back to Alsophila

Pi 0.051

MarkerSeek score 71.60

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: Genome
length_bp: 153
variable_sites: 14
pi: 0.051
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CGTTAGCCGAGGTACGTTAC	TGAAACCACTCGCAGTATGG	55.0	50.0	58.2	57.9	205-211 bp	1.0	85.4
ndhD-psaC_p2	AGCCGAGGTACGTTACTCAT	TGAAACCACTCGCAGTATGG	50.0	50.0	58.2	57.9	201-207 bp	1.0	85.1
ndhD-psaC_p3	TCGTTAGCCGAGGTACGTTA	TGAAACCACTCGCAGTATGG	50.0	50.0	58.3	57.9	206-212 bp	1.0	84.7
ndhD-psaC_p4	CGTTAGCCGAGGTACGTTAC	GCTGAAACCACTCGCAGTAT	55.0	50.0	58.2	58.3	207-213 bp	1.0	83.9
ndhD-psaC_p5	TAGCCGAGGTACGTTACTCA	TGAAACCACTCGCAGTATGG	50.0	50.0	57.6	57.9	202-208 bp	1.0	83.8