Hotspot

ccsA-ndhD

Genome · 116098-116516 · 419 bp

Back to Alsophila

Pi 0.0621

MarkerSeek score 64.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: Genome
length_bp: 419
variable_sites: 44
pi: 0.0621
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GTATTCTTCCCGGAGCAGTG	TGGTATTCCCAGGAGTCGTT	55.0	50.0	58.1	58.1	969-978 bp	1.0	85.4
ccsA-ndhD_p2	GTATTCTTCCCGGAGCAGTG	TTGGTATTCCCAGGAGTCGT	55.0	50.0	58.1	58.1	970-979 bp	1.0	85.4
ccsA-ndhD_p3	GAGCAGTGTGGGCTAATGAA	TGGTATTCCCAGGAGTCGTT	50.0	50.0	57.9	58.1	957-966 bp	1.0	85.0
ccsA-ndhD_p4	GTATTCTTCCCGGAGCAGTG	CTCGACCAACTAGGAGGGAT	55.0	55.0	58.1	57.9	1084-1093 bp	1.0	84.7
ccsA-ndhD_p5	GTATTCTTCCCGGAGCAGTG	CTTGCGGGTACTTGTTACGA	55.0	50.0	58.1	57.9	1123-1132 bp	1.0	84.6