Hotspot

rpl32-trnP-GGG

Genome · 113932-114558 · 627 bp

Back to Alsophila

Pi 0.053

MarkerSeek score 76.20

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: Genome
length_bp: 627
variable_sites: 56
pi: 0.053
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	AAACAACTCCGAGTGGAACC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1593-1622 bp	1.0	88.0
rpl32-trnP-GGG_p2	AAACAACTCCGAGTGGAACC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1594-1623 bp	1.0	88.0
rpl32-trnP-GGG_p3	ATTGACGTTGGTGGGAAACA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	1608-1637 bp	1.0	87.8
rpl32-trnP-GGG_p4	AAACAACTCCGAGTGGAACC	CAATGCGGCAAAGTAACAGG	50.0	50.0	58.0	58.0	1959-1995 bp	1.0	87.2
rpl32-trnP-GGG_p5	AAACAACTCCGAGTGGAACC	CCAATGCGGCAAAGTAACAG	50.0	50.0	58.0	58.0	1960-1996 bp	1.0	87.2