Hotspot

rpl23-trnI-CAU

Genome · 85981-86205 · 225 bp

Back to Alsophila

Pi 0.0702

MarkerSeek score 68.50

Primer available yes

Label rpl23-trnI-CAU

Feature Metadata

label_name: rpl23-trnI-CAU
feature_type: igs
region: Genome
length_bp: 225
variable_sites: 27
pi: 0.0702
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-trnI-CAU_p1	ACAATCATTCTTTTGCGGCG	TGGCGAATTCACAGGTTCAA	45.0	45.0	58.0	58.0	492-499 bp	1.0	86.6
rpl23-trnI-CAU_p2	ACAATCATTCTTTTGCGGCG	TTGGCGAATTCACAGGTTCA	45.0	45.0	58.0	58.0	493-500 bp	1.0	86.6
rpl23-trnI-CAU_p3	CAATCATTCTTTTGCGGCGT	TGGCGAATTCACAGGTTCAA	45.0	45.0	58.0	58.0	491-498 bp	1.0	86.6
rpl23-trnI-CAU_p4	CAATCATTCTTTTGCGGCGT	TTGGCGAATTCACAGGTTCA	45.0	45.0	58.0	58.0	492-499 bp	1.0	86.6
rpl23-trnI-CAU_p5	TCAGAGGTAGGGCATTTCCA	TGGCGAATTCACAGGTTCAA	50.0	45.0	58.0	58.0	993-1004 bp	1.0	85.1