Hotspot

petG-trnW-CCA

Genome · 65407-65726 · 320 bp

Back to Alsophila

Pi 0.0581

MarkerSeek score 73.20

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: Genome
length_bp: 320
variable_sites: 32
pi: 0.0581
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACGCGGTGACCAATTAGAAA	GTTCGAATCCTACAGGGCG	45.0	57.9	57.8	58.0	366-371 bp	1.0	78.6
petG-trnW-CCA_p2	ATTCACAATATCGACGCGGT	GTTCGAATCCTACAGGGCG	45.0	57.9	57.8	58.0	379-384 bp	1.0	78.0
petG-trnW-CCA_p3	GACGCGGTGACCAATTAGAA	GTTCGAATCCTACAGGGCG	50.0	57.9	58.3	58.0	367-372 bp	1.0	77.7
petG-trnW-CCA_p4	ACGCGGTGACCAATTAGAAA	AGGTTCGAATCCTACAGGGC	45.0	55.0	57.8	59.2	368-373 bp	1.0	77.5
petG-trnW-CCA_p5	ACGCGGTGACCAATTAGAAA	GGGTCTCCAAAACCCGATG	45.0	57.9	57.8	58.1	391-396 bp	1.0	77.3