Hotspot

psbE-petL

Genome · 64046-65041 · 996 bp

Back to Alsophila

Pi 0.0477

MarkerSeek score 65.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 996
variable_sites: 80
pi: 0.0477
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAGCCAACCTGCAATAAACG	AGAAGGGTCTGCGGACTAAT	50.0	50.0	58.0	58.1	1674-1679 bp	0.6	63.2
psbE-petL_p2	CAGCCAACCTGCAATAAACG	TCCTACAGGGCGTGTCTATT	50.0	50.0	58.0	57.8	1803-1808 bp	0.6	63.0
psbE-petL_p3	CAGCCAACCTGCAATAAACG	ATGGGGCCCTCCTTCTTTAT	50.0	50.0	58.0	58.1	1254 bp	0.4	55.3
psbE-petL_p4	CCTATCACAAGTGTACCGGC	ATGGGGCCCTCCTTCTTTAT	55.0	50.0	58.1	58.1	1856 bp	0.4	54.9
psbE-petL_p5	GCCTATCACAAGTGTACCGG	ATGGGGCCCTCCTTCTTTAT	55.0	50.0	58.1	58.1	1857 bp	0.4	54.9