Hotspot

petA-psbJ

Genome · 62508-63280 · 773 bp

Back to Alsophila

Pi 0.0388
MarkerSeek score 67.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
Genome
length_bp
773
variable_sites
50
pi
0.0388
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGTGGGTGGATTTGGTCAAG CGTTTATTGCAGGTTGGCTG 50.0 50.0 58.0 58.0 1560-1616 bp 1.0 86.9
petA-psbJ_p2 TTTGAGAAGGTCCAGTTGGC CGTTTATTGCAGGTTGGCTG 50.0 50.0 58.0 58.0 1433-1489 bp 1.0 86.9
petA-psbJ_p3 TCTGTTAAAGCCGATCAGCC CGTTTATTGCAGGTTGGCTG 50.0 50.0 58.0 58.0 1598-1654 bp 1.0 86.6
petA-psbJ_p4 GCCGATCAGCCATTGACTAA CGTTTATTGCAGGTTGGCTG 50.0 50.0 58.0 58.0 1589-1645 bp 1.0 86.5
petA-psbJ_p5 CTATCGCCCGGACAAAAAGA CGTTTATTGCAGGTTGGCTG 50.0 50.0 58.0 58.0 1947-2003 bp 1.0 86.4