Hotspot

trnC-GCA-trnG-GCC

Genome · 30462-31972 · 1511 bp

Back to Alsophila

Pi 0.167
MarkerSeek score 68.40
Primer available yes
Label trnC-GCA-trnG-GCC

Feature Metadata

label_name
trnC-GCA-trnG-GCC
feature_type
igs
region
Genome
length_bp
1511
variable_sites
409
pi
0.167
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.83
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-trnG-GCC_p1 CAGATTCGAACTGGGGGATG GTTCGATTCCCGCTATCCG 55.0 57.9 58.0 58.2 1081-1594 bp 1.0 82.2
trnC-GCA-trnG-GCC_p2 AGGATTTGCAATCCTCCGTC GTTCGATTCCCGCTATCCG 50.0 57.9 58.0 58.2 1060-1573 bp 1.0 82.1
trnC-GCA-trnG-GCC_p3 GGATTTGCAATCCTCCGTCT GTTCGATTCCCGCTATCCG 50.0 57.9 58.0 58.2 1059-1572 bp 1.0 82.1
trnC-GCA-trnG-GCC_p4 CAGATTCGAACTGGGGGATG GGGTTCGATTCCCGCTATC 55.0 57.9 58.0 57.8 1083-1596 bp 1.0 82.0
trnC-GCA-trnG-GCC_p5 CAGATTCGAACTGGGGGATG GGTTCGATTCCCGCTATCC 55.0 57.9 58.0 57.8 1082-1595 bp 1.0 82.0