Hotspot

rpoB-trnD-GUC

Genome · 26509-27666 · 1158 bp

Back to Alsophila

Pi 0.0489

MarkerSeek score 75.60

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: Genome
length_bp: 1158
variable_sites: 97
pi: 0.0489
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	GAATTCGTGCAAGTTCTGGC	AACCTCGCGATCGTACTAGA	50.0	50.0	58.0	58.1	1562-1620 bp	1.0	87.6
rpoB-trnD-GUC_p2	GAATTCGTGCAAGTTCTGGC	ACCTCGCGATCGTACTAGAA	50.0	50.0	58.0	58.1	1561-1619 bp	1.0	87.6
rpoB-trnD-GUC_p3	GAATTCGTGCAAGTTCTGGC	AGAACCTCGCGATCGTACTA	50.0	50.0	58.0	58.1	1564-1622 bp	1.0	87.6
rpoB-trnD-GUC_p4	GAATTCGTGCAAGTTCTGGC	GATTGACGATTCGTGGGGAA	50.0	50.0	58.0	58.0	1696-1754 bp	1.0	87.4
rpoB-trnD-GUC_p5	TTGATCCGATTCGCGATTGT	GATTGACGATTCGTGGGGAA	45.0	50.0	58.1	58.0	2061-2119 bp	1.0	85.9