Hotspot

atpH-atpI

Genome · 13486-14240 · 755 bp

Back to Alsophila

Pi 0.0232

MarkerSeek score 66.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 755
variable_sites: 30
pi: 0.0232
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCAGCAATAACAGAAGCAGC	GGGAATCTACGGAGGGTCAT	50.0	55.0	58.1	58.3	810-819 bp	1.0	82.0
atpH-atpI_p2	AGAAGCAGCAGGAATCAAGG	GGGAATCTACGGAGGGTCAT	50.0	55.0	57.9	58.3	799-808 bp	1.0	81.8
atpH-atpI_p3	GCAGCAATAACAGAAGCAGC	TACAGGGGAATCTACGGAGG	50.0	55.0	58.1	57.6	815-824 bp	1.0	81.2
atpH-atpI_p4	AGAAGCAGCAGGAATCAAGG	TACAGGGGAATCTACGGAGG	50.0	55.0	57.9	57.6	804-813 bp	1.0	81.0
atpH-atpI_p5	CAGCAGGAATCAAGGGGTTC	GGGAATCTACGGAGGGTCAT	55.0	55.0	58.5	58.3	794-803 bp	1.0	78.6