Hotspot

rpoC2

Genome · 17562-21719 · 4158 bp

Back to Alseodaphnopsis

Pi 0.00189

MarkerSeek score 48.30

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: Genome
length_bp: 4158
variable_sites: 20
pi: 0.00189
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AATCGTGAGGGAGAAAGCAC	TTCGTTGGGTCCTTTAGGGA	50.0	50.0	57.9	58.3	245 bp	1.0	78.0
rpoC2_p2	AATCGTGAGGGAGAAAGCAC	GATCCCGCGATTCCCATTAG	50.0	55.0	57.9	58.3	268 bp	1.0	77.9
rpoC2_p3	GTGAGGGAGAAAGCACCAAT	TTCGTTGGGTCCTTTAGGGA	50.0	50.0	57.8	58.3	241 bp	1.0	77.2
rpoC2_p4	GTGAGGGAGAAAGCACCAAT	GATCCCGCGATTCCCATTAG	50.0	55.0	57.8	58.3	264 bp	1.0	77.1
rpoC2_p5	AATCGTGAGGGAGAAAGCAC	ATTCGTTGGGTCCTTTAGGG	50.0	50.0	57.9	57.2	246 bp	1.0	73.8