Hotspot

rpl32-trnL-UAG

Genome · 117440-118822 · 1383 bp

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Pi 0.00834

MarkerSeek score 64.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: Genome
length_bp: 1383
variable_sites: 29
pi: 0.00834
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGCGGCAAAAGCTTTATCTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.7	57.9	1512-1524 bp	1.0	79.0
rpl32-trnL-UAG_p2	CGCGGCAAAAGCTTTATCTT	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.7	57.8	1505-1517 bp	1.0	78.5
rpl32-trnL-UAG_p3	CGCGGCAAAAGCTTTATCTT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.7	57.8	1511-1523 bp	1.0	78.4
rpl32-trnL-UAG_p4	CGCGGCAAAAGCTTTATCTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.7	57.8	1510-1522 bp	1.0	78.4
rpl32-trnL-UAG_p5	CGCGGCAAAAGCTTTATCTT	GAGCAGCGTGTCTACCAATT	45.0	50.0	57.7	58.3	1506-1518 bp	1.0	77.8