Hotspot

ndhF-rpl32

Genome · 116039-117265 · 1227 bp

Back to Alseodaphnopsis

Pi 0.00924

MarkerSeek score 65.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 1227
variable_sites: 27
pi: 0.00924
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAACAAGCAGAAGTCCCAA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1330-1361 bp	1.0	79.6
ndhF-rpl32_p2	AGCAGAAGTCCCAACCCTAT	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1324-1355 bp	1.0	79.6
ndhF-rpl32_p3	AAGCAGAAGTCCCAACCCTA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.3	57.7	1325-1356 bp	1.0	77.7
ndhF-rpl32_p4	GGAACAAGCAGAAGTCCCAA	GGCCTGATATCCCCTTCCTT	50.0	55.0	58.0	58.5	1388-1419 bp	1.0	77.6
ndhF-rpl32_p5	AGCAGAAGTCCCAACCCTAT	GGCCTGATATCCCCTTCCTT	50.0	55.0	58.0	58.5	1382-1413 bp	1.0	77.5