Hotspot

psbE-petL

Genome · 68325-69574 · 1250 bp

Back to Alseodaphnopsis

Pi 0.00278

MarkerSeek score 55.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 1250
variable_sites: 8
pi: 0.00278
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1427-1449 bp	1.0	77.1
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1425-1447 bp	1.0	77.0
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.2	58.5	1436-1458 bp	1.0	76.1
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GCAGCCAGTAGAAAACCGAA	45.0	50.0	58.2	58.5	1434-1456 bp	1.0	76.1
psbE-petL_p5	TGCTGACGAATAACCAACCC	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.2	58.5	1441-1463 bp	1.0	76.0