Hotspot

ycf4-cemA

Genome · 63620-64530 · 911 bp

Back to Alseodaphnopsis

Pi 0.00439

MarkerSeek score 55.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: Genome
length_bp: 911
variable_sites: 9
pi: 0.00439
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AAAAAGCTGCTGAATTGGCC	AGATCCACCAGGGCAAAAAG	45.0	50.0	57.8	58.1	1017-1036 bp	1.0	80.4
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	1018-1037 bp	1.0	80.3
ycf4-cemA_p3	AAAAAGCTGCTGAATTGGCC	GATCCACCAGGGCAAAAAGA	45.0	50.0	57.8	58.1	1016-1035 bp	1.0	80.3
ycf4-cemA_p4	AAAGCTGCTGAATTGGCCTA	AGATCCACCAGGGCAAAAAG	45.0	50.0	57.8	58.1	1015-1034 bp	1.0	80.0
ycf4-cemA_p5	AAAGCTGCTGAATTGGCCTA	GATCCACCAGGGCAAAAAGA	45.0	50.0	57.8	58.1	1014-1033 bp	1.0	80.0