Hotspot

trnS-GCU-trnG-UCC

Genome · 9522-10345 · 824 bp

Back to Alseodaphnopsis

Pi 0.00465

MarkerSeek score 56.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: Genome
length_bp: 824
variable_sites: 10
pi: 0.00465
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACGCTTTTACCACT	45.0	42.9	58.0	58.2	892-898 bp	1.0	71.2
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	ACGAATCACGCTTTTACCAC	45.0	45.0	58.0	56.8	892-898 bp	1.0	71.2
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	AACGAATCACGCTTTTACCAC	45.0	42.9	58.0	57.5	893-899 bp	1.0	68.9
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	CGAATCACGCTTTTACCACT	45.0	45.0	58.0	56.5	891-897 bp	1.0	68.9
trnS-GCU-trnG-UCC_p5	GCTTTAGTCCACTCAGCCAT	ACGAATCACGCTTTTACCACT	50.0	42.9	57.6	58.2	878-884 bp	1.0	68.3