Hotspot

ycf1

SSC · 128862-134447 · 5586 bp

Back to Alseodaphne

Pi 0.00274

MarkerSeek score 54.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5586
variable_sites: 23
pi: 0.00274
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTTCGCGATATTGAAGTAGA	GAGTCAATGTACAACTTTTCCTTGA	40.9	36.0	57.7	57.7	278 bp	1.0	41.2
ycf1_p2	TGCTTCGCGATATTGAAGTAGA	AGTCAATGTACAACTTTTCCTTGA	40.9	33.3	57.7	56.7	277 bp	1.0	41.2
ycf1_p3	TGCTTCGCGATATTGAAGTAGA	AGAGTCAATGTACAACTTTTCCT	40.9	34.8	57.7	55.8	279 bp	1.0	41.1
ycf1_p4	TGCTTCGCGATATTGAAGTAGA	AGAGTCAATGTACAACTTTTCCTTG	40.9	36.0	57.7	57.7	279 bp	1.0	41.1
ycf1_p5	ATGCTTCGCGATATTGAAGT	AGAGTCAATGTACAACTTTTCCT	40.0	34.8	55.6	55.8	280 bp	1.0	41.1