Hotspot

ccsA

SSC · 119337-120287 · 951 bp

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Pi 0.0021

MarkerSeek score 51.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 951
variable_sites: 3
pi: 0.0021
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGGCGGCATCCTCTAAAAA	TCCTATGTATTCTTCAGGCCCT	50.0	45.5	58.5	58.1	1073 bp	1.0	59.4
ccsA_p2	GTGGCGGCATCCTCTAAAAA	TGTATTCTTCAGGCCCTTTCT	50.0	42.9	58.5	56.6	1068 bp	1.0	56.9
ccsA_p3	GTGGCGGCATCCTCTAAAAA	TCCTATGTATTCTTCAGGCCC	50.0	47.6	58.5	56.6	1073 bp	1.0	56.5
ccsA_p4	GTGGCGGCATCCTCTAAAAA	CCTATGTATTCTTCAGGCCCT	50.0	47.6	58.5	56.6	1072 bp	1.0	56.4
ccsA_p5	GTGGCGGCATCCTCTAAAA	TCCTATGTATTCTTCAGGCCCT	52.6	45.5	57.8	58.1	1073 bp	1.0	54.4