Hotspot

ccsA-ndhD

SSC · 120288-120523 · 236 bp

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Pi 0.0198

MarkerSeek score 62.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 236
variable_sites: 7
pi: 0.0198
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GTGGATTCGGCAATTGTGG	TCCTGATTTCGTTCTCTCGC	52.6	50.0	57.3	57.7	424-426 bp	1.0	65.7
ccsA-ndhD_p2	GTGGATTCGGCAATTGTGG	CCTGATTTCGTTCTCTCGCT	52.6	50.0	57.3	57.7	423-425 bp	1.0	65.7
ccsA-ndhD_p3	TGGATTCGGCAATTGTGGC	TCCTGATTTCGTTCTCTCGC	52.6	50.0	59.1	57.7	423-425 bp	1.0	62.6
ccsA-ndhD_p4	TGGATTCGGCAATTGTGGC	CCTGATTTCGTTCTCTCGCT	52.6	50.0	59.1	57.7	422-424 bp	1.0	62.5
ccsA-ndhD_p5	GCAATTGTGGCTTCTATAGGG	TCCTGATTTCGTTCTCTCGC	47.6	50.0	56.7	57.7	415-417 bp	1.0	61.3