Hotspot

rpl32-trnL-UAG

SSC · 117797-119169 · 1373 bp

Back to Alseodaphne

Pi 0.00583
MarkerSeek score 61.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1373
variable_sites
12
pi
0.00583
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGCGGCAAAAGCTTTATCTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.7 57.9 1488-1506 bp 1.0 78.5
rpl32-trnL-UAG_p2 CGCGGCAAAAGCTTTATCTT AGCAGCGTGTCTACCAATTT 45.0 45.0 57.7 57.8 1481-1499 bp 1.0 78.0
rpl32-trnL-UAG_p3 CGCGGCAAAAGCTTTATCTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.7 57.8 1487-1505 bp 1.0 77.9
rpl32-trnL-UAG_p4 CGCGGCAAAAGCTTTATCTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.7 57.8 1486-1504 bp 1.0 77.9
rpl32-trnL-UAG_p5 CGCGGCAAAAGCTTTATCTT GAGCAGCGTGTCTACCAATT 45.0 50.0 57.7 58.3 1482-1500 bp 1.0 77.3