Hotspot

accD-psaI

LSC · 62057-62641 · 585 bp

Back to Alseodaphne

Pi 0.00684

MarkerSeek score 62.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 585
variable_sites: 6
pi: 0.00684
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTACACGGTTTCTTTCCC	GCCATTGCAATTGCTGGAAA	50.0	45.0	57.8	58.2	730 bp	1.0	77.7
accD-psaI_p2	GCTACACGGTTTCTTTCCCT	GCCATTGCAATTGCTGGAAA	50.0	45.0	57.8	58.2	729 bp	1.0	77.7
accD-psaI_p3	AGCTACACGGTTTCTTTCCC	AGAAGCCATTGCAATTGCTG	50.0	45.0	57.8	57.6	734 bp	1.0	76.2
accD-psaI_p4	GCTACACGGTTTCTTTCCCT	AGAAGCCATTGCAATTGCTG	50.0	45.0	57.8	57.6	733 bp	1.0	76.2
accD-psaI_p5	GCTACACGGTTTCTTTCCCT	TTGCTGGAAACACTAAGCCC	50.0	50.0	57.8	58.4	719 bp	1.0	76.2