Hotspot

trnS-GCU-trnG-UCC

LSC · 9560-10386 · 827 bp

Back to Alseodaphne

Pi 0.00404

MarkerSeek score 61.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 5
pi: 0.00404
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACGCTTTTACCACT	45.0	42.9	58.0	58.2	898-901 bp	1.0	70.5
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	ACGAATCACGCTTTTACCAC	45.0	45.0	58.0	56.8	898-901 bp	1.0	70.5
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	AACGAATCACGCTTTTACCAC	45.0	42.9	58.0	57.5	899-902 bp	1.0	68.2
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	CGAATCACGCTTTTACCACT	45.0	45.0	58.0	56.5	897-900 bp	1.0	68.1
trnS-GCU-trnG-UCC_p5	GCTTTAGTCCACTCAGCCAT	ACGAATCACGCTTTTACCACT	50.0	42.9	57.6	58.2	884-887 bp	1.0	67.5