Hotspot

matK-trnK-UUU

LSC · 3657-4375 · 719 bp

Back to Alseodaphne

Pi 0.00279

MarkerSeek score 60.70

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 3
pi: 0.00279
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TTGTTGCCGAAATCCATCCA	GTCGGGCAATCGAATCTCTT	45.0	50.0	58.1	58.1	867-868 bp	1.0	79.2
matK-trnK-UUU_p2	TGTTGCCGAAATCCATCCAT	GTCGGGCAATCGAATCTCTT	45.0	50.0	57.9	58.1	866-867 bp	1.0	78.8
matK-trnK-UUU_p3	TTGTTGCCGAAATCCATCCA	AACGGTAGAGTACTCGGCTT	45.0	50.0	58.1	58.2	790-791 bp	1.0	78.4
matK-trnK-UUU_p4	TTGTTGCCGAAATCCATCCA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	58.1	58.2	789-790 bp	1.0	78.4
matK-trnK-UUU_p5	TTGTTGCCGAAATCCATCCA	ATGGGTTGCTAACTCAACGG	45.0	50.0	58.1	58.2	805-806 bp	1.0	78.3