Hotspot

ycf1

SSC · 128721-134177 · 5457 bp

Back to Alpinia

Pi 0.0127
MarkerSeek score 51.10
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5457
variable_sites
189
pi
0.0127
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TGTGTGTTGAAATTATATGGAAT ACAGTGTATTTATGGCCGAA 26.1 40.0 52.4 54.8 170-188 bp 0.25 12.2
ycf1_p2 TGTGTGTTGAAATTATATGGAAT ACAGTGTATTTATGGCCGAAT 26.1 38.1 52.4 55.4 170-188 bp 0.25 12.2
ycf1_p3 TGTGTGTTGAAATTATATGGAAT TACAGTGTATTTATGGCCGA 26.1 40.0 52.4 53.9 171-189 bp 0.25 12.2
ycf1_p4 TGTGTGTTGAAATTATATGGAAT TACAGTGTATTTATGGCCGAA 26.1 38.1 52.4 54.7 171-189 bp 0.25 12.2
ycf1_p5 TGTGTGTTGAAATTATATGGAAT ATACAGTGTATTTATGGCCGA 26.1 38.1 52.4 54.5 172-190 bp 0.25 12.2