Hotspot

atpF

LSC · 11659-13053 · 1395 bp

Back to Alpinia

Pi 0.0172

MarkerSeek score 60.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1395
variable_sites: 64
pi: 0.0172
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CAATTCCGTCGCCTACTTGA	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.0	58.0	2095-2151 bp	1.0	87.3
atpF_p2	CAATTCCGTCGCCTACTTGA	AGGCCAAGCTGTAGAAGGTA	50.0	50.0	58.0	58.0	2202-2258 bp	1.0	86.6
atpF_p3	CCGTTTCGGCTACCACAATA	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.0	58.0	2662-2718 bp	1.0	85.8
atpF_p4	CAATTCCGTCGCCTACTTGA	AGGAATTACCGCATTCGCTT	50.0	45.0	58.0	58.0	1888-1937 bp	1.0	85.3
atpF_p5	CCGTTTCGGCTACCACAATA	AGGAATTACCGCATTCGCTT	50.0	45.0	58.0	58.0	2455-2504 bp	1.0	84.2