Hotspot

trnN-GUU-trnR-ACG

IRa · 134566-135138 · 573 bp

Back to Alpinia

Pi 0.0147

MarkerSeek score 69.20

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 573
variable_sites: 23
pi: 0.0147
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	678 bp	1.0	83.8
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	665 bp	1.0	83.8
trnN-GUU-trnR-ACG_p3	CTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.8	57.8	666 bp	1.0	83.4
trnN-GUU-trnR-ACG_p4	GCTGTTAACTGACTGGTCGT	GAACAGATCCAGTGGAGACG	50.0	55.0	57.8	57.7	715 bp	1.0	83.1
trnN-GUU-trnR-ACG_p5	TGGTCGTAGGTTCGAATCCT	GAACAGATCCAGTGGAGACG	50.0	55.0	58.2	57.7	702 bp	1.0	83.1