Hotspot

rpl32-trnL-UAG

SSC · 117896-118782 · 887 bp

Back to Alpinia

Pi 0.0392

MarkerSeek score 78.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 887
variable_sites: 86
pi: 0.0392
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGTTTCTACCGGACGTTCAA	TCAGGCAATCCTACGCTTTC	50.0	50.0	57.8	58.0	1194-1241 bp	1.0	89.4
rpl32-trnL-UAG_p2	GGTTTCTACCGGACGTTCAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	943-990 bp	1.0	89.4
rpl32-trnL-UAG_p3	GGTTTCTACCGGACGTTCAA	GTCCCGAAGAAATCCAACGA	50.0	50.0	57.8	57.9	1271-1318 bp	1.0	88.6
rpl32-trnL-UAG_p4	GGTTTCTACCGGACGTTCAA	ACGGATTGGTGCATTTCAGT	50.0	45.0	57.8	58.1	1452-1499 bp	1.0	87.8
rpl32-trnL-UAG_p5	GGTTTCTACCGGACGTTCAA	TGAATCAGGCAATCCTACGC	50.0	50.0	57.8	58.0	1198-1245 bp	0.875	83.3