Hotspot

accD-psaI

LSC · 62185-62705 · 521 bp

Back to Alpinia

Pi 0.0315

MarkerSeek score 74.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 521
variable_sites: 38
pi: 0.0315
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACTCCACGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	611-661 bp	1.0	88.4
accD-psaI_p2	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	607-657 bp	1.0	88.4
accD-psaI_p3	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	608-658 bp	1.0	88.4
accD-psaI_p4	TCGACCCAATAGTACCACGT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	665-715 bp	1.0	86.9
accD-psaI_p5	CTCCACGGTTTCTTTCCCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.7	58.0	609-659 bp	1.0	86.4