Hotspot

ndhC-trnV-UAC

LSC · 52789-54483 · 1695 bp

Back to Alpinia

Pi 0.0225
MarkerSeek score 70.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1695
variable_sites
99
pi
0.0225
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.91
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCGGAAATTACAAATGCCA AGAAGGTCTACGGTTCGAGT 40.0 50.0 55.6 57.8 1630-1858 bp 1.0 67.9
ndhC-trnV-UAC_p2 CCGGAAATTACAAATGCCAAGA AGAAGGTCTACGGTTCGAGT 40.9 50.0 57.6 57.8 1629-1857 bp 1.0 67.4
ndhC-trnV-UAC_p3 TCCGGAAATTACAAATGCCAAG AGAAGGTCTACGGTTCGAGT 40.9 50.0 57.6 57.8 1630-1858 bp 1.0 67.4
ndhC-trnV-UAC_p4 TCCGGAAATTACAAATGCCAA AGAAGGTCTACGGTTCGAGT 38.1 50.0 56.4 57.8 1630-1858 bp 1.0 66.2
ndhC-trnV-UAC_p5 TCCGGAAATTACAAATGCCA GAAGGTCTACGGTTCGAGTC 40.0 55.0 55.6 57.5 1629-1857 bp 1.0 65.1