Hotspot

trnG-GCC-trnfM-CAU

LSC · 38717-38909 · 193 bp

Back to Alpinia

Pi 0.0316

MarkerSeek score 72.30

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 193
variable_sites: 17
pi: 0.0316
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	240-260 bp	1.0	88.7
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	241-261 bp	1.0	88.6
trnG-GCC-trnfM-CAU_p3	AGAAGATGCGGGTTCGATTC	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	234-254 bp	1.0	88.2
trnG-GCC-trnfM-CAU_p4	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	281-301 bp	1.0	87.6
trnG-GCC-trnfM-CAU_p5	TGTGTGGACATATGCATGCA	CTTGAGGTCACGGGTTCAAA	45.0	50.0	57.9	58.0	320-340 bp	1.0	86.8