Hotspot

atpH-atpI

LSC · 13830-15062 · 1233 bp

Back to Alpinia

Pi 0.0207

MarkerSeek score 65.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1233
variable_sites: 70
pi: 0.0207
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AAGCAGCAGAAATCAGTGGA	ATTTTTGCAACCTTAGCCGC	45.0	45.0	57.4	57.9	1196-1315 bp	1.0	83.3
atpH-atpI_p2	AGCAGCAGAAATCAGTGGAT	ATTTTTGCAACCTTAGCCGC	45.0	45.0	57.2	57.9	1195-1314 bp	1.0	81.5
atpH-atpI_p3	GAAGCAGCAGAAATCAGTGG	ATTTTTGCAACCTTAGCCGC	50.0	45.0	57.1	57.9	1197-1316 bp	1.0	80.6
atpH-atpI_p4	AAGCAGCAGAAATCAGTGGAT	ATTTTTGCAACCTTAGCCGC	42.9	45.0	57.9	57.9	1196-1315 bp	1.0	79.2
atpH-atpI_p5	AGCAGCAGAAATCAGTGGATT	ATTTTTGCAACCTTAGCCGC	42.9	45.0	57.9	57.9	1195-1314 bp	1.0	79.2