Hotspot

ndhF-rpl32

SSC · 104361-105259 · 899 bp

Back to Alopecurus

Pi 0.0185

MarkerSeek score 63.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 899
variable_sites: 36
pi: 0.0185
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TGCTAAAGAATAAGCGTGTACT	55.0	36.4	58.1	55.6	1028-1055 bp	1.0	48.7
ndhF-rpl32_p2	GGCCAAACCCCATTGACATA	TGCTAAAGAATAAGCGTGTACT	50.0	36.4	58.1	55.6	1054-1081 bp	1.0	48.4
ndhF-rpl32_p3	AAAAGGCCAAACCCCATTGA	TGCTAAAGAATAAGCGTGTACT	45.0	36.4	58.2	55.6	1058-1085 bp	1.0	48.0
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTGCTAAAGAATAAGCGTGTACT	55.0	34.8	58.1	56.3	1029-1056 bp	1.0	46.5
ndhF-rpl32_p5	GGCCAAACCCCATTGACATA	TTGCTAAAGAATAAGCGTGTACT	50.0	34.8	58.1	56.3	1055-1082 bp	1.0	46.1